ABSTRACT
Stroke is a devastating cerebrovascular event that occurs as a result of cerebral vascular occlusion (ischemic stroke) or burst/bleeding (hemorrhagic stroke) leading to cerebral blood flow disruption, causing physical disability and multiple functional impairment. The incidence of ischemic stroke is higher than hemorrhagic stroke, accounting for more than half of the total incidence of stroke. The main cause of the disease is caused by atherosclerosis, various arteritis, trauma and other plaques that cause cerebral vascular disease, which may be caused by a variety of cardiovascular diseases such as atrial fibrillation. Blood, caused by blocking blood vessels in the brain. Due to the complexity of the causes of ischemic stroke, the pathogenesis and development mechanism are complex and diverse, such as energy failure, excitotoxicity, neuroinflammation, apoptosis and oxidative stress. Traditional Chinese medicine and compound have the characteristics of multi-component, multi-target, multi-effect, and can be applied to the treatment of different stages of stroke occurrence and development. Therefore, an in-depth understanding of the pathological process and related mechanisms of ischemic stroke, and the development of targeted drugs, is important to prevent the occurrence of ischemic stroke and control its development. In this paper, the timeline of the occurrence and development of ischemic stroke is the main line. The pathological process and related mechanisms are systematically reviewed. The most widely used traditional Chinese medicine and compound in clinical and basic research are summarized, in order to study the pathological study of ischemic stroke. Clinical treatment provides a theoretical basis and data reference.
ABSTRACT
Objective: The effect of Liuwei Dihuangwan on blood metabolism during growth and development of rats was investigated by taking the overall metabolic profile and biomarkers of metabolomics as indexes. Method: Ultra performance liquid chromatography-high definition mass spectrometry(UPLC-HDMS) was employed to establish a blood metabolomics study method for characterizing the blood metabolic profile of rats at different time before and after administration. The pattern recognition method was used to integrate and analyze the metabolic profiles, and the differential metabolic markers related to drug action were searched. Based on metabolomics pathway analysis(MetPA) and Kyoto encyclopedia of genes and genomes(KEGG) and other databases, the metabolic pathways related to differential markers were analyzed to study the effect of Liuwei Dihuangwan on blood metabolism during growth and development of rats. Result: Liuwei Dihuangwan significantly affect the blood metabolism profiles during growth and development of rats by regulating 30 blood metabolic markers and 12 related target metabolic pathways, and 8 key metabolic markers were delineated. Conclusion: Liuwei Dihuangwan can significantly regulate the blood metabolic network during the growth and development of rats, thereby affecting the growth and development of rats.
ABSTRACT
On base of traditional physiological studies, building a comprehensive evaluation system for the efficacy of Liuwei Dihuang Wan (LW) for regulating the growth and development of rats by indicators that the overall metabolic profile and biomarkers of urinary metabonomics. Sixty-seven day-old Sprague-Dawley rats were randomly divided into four groups that are the control group and low-dose, medium-dose, and high-dose groups of LW. At the age of 28 days, different doses of LW extracts were continuously administered for 16 days. On the basis of behavior, body weight, organ index and femur length, a UPLC-HDMS technique was used to establish urine metabolomics method to characterize the profile of urine metabolism before and after intervention of LW, analysis and identificate related to drug effects of differential metabolism markers, and study the effect of LW on the growth and development of rats. LW could improve the overall behavioral ability of rats, promote weight gain and the growth of organs and bones, and 36 differential metabolites and 13 target metabolic pathways of urine under the intervention of LW were identified, and 8 metabolic markers were regarded as a key marker that LW effect the growth and development of rats. LW has a significant regulatory effect on the urine metabolic network during the growth and development of rats. This study interpreted the scientific implications of the effect of "Nourishing Kidney Yin" on the growth and development of rats.